Descriptions and Lists from
the VIDE Database
mottle mosaic tobamovirus
Data collated by R.I.B. Francki, 1988.
cucumber green mottle mosaic virus, tobacco mosaic
virus watermelon strain - W, cucumber virus 3 (Ainsworth, 1935), cucumber
virus 4 (Ainsworth, 1935; Hollings et al., 1975), bottlegourd Indian
mosaic virus, cucumis virus 2.
ICTV decimal code
Host range and symptoms First reported
in Cucumis sativus; from Great Britain; by Ainsworth (1935).
Natural host range and symptoms
- Cucumis sativus, Citrullus vulgaris, Lagenaria siceraria
(bottlegourd) - mosaic.
Transmitted by means not involving a vector,
or a vector; an insect; Raphidopalpa fevicolis (Rao and Varma, 1984);
Coleoptera. Virus transmitted by mechanical inoculation.
Spreads in the Eurasian region; India, Japan, and the UK.
Experimental host range
Few (<3) families susceptible.
Diagnostically susceptible host species and symptoms
- Cucumis sativus, Citrullus vulgaris - systemic mosaic.
Diagnostically insusceptible host species
stramonium, Petunia × hybrida.
Maintenance and propagation
Assay hosts (Local lesions or Whole plants)
Chenopodium amaranticolor (L), Cucumis sativus (W).
Susceptible host species
Insusceptible host species
Families containing susceptible hosts
Properties of particles in sap
80-90 °C. LIV: more than 240 days (at 20ºC). DEP: log10 minus 6. Leaf
sap contains many virions.
Tung and Knight
(1972); Francki and McLean (1968).
Virions rod-shaped; not enveloped;
usually straight; with a clear modal length; of c 300 nm; c. 15 nm
wide. Axial canal obvious. Basic helix obvious.
Virions contain c. 5 %
nucleic acid; c. 95 % protein.
Genome consists of RNA; single-stranded; linear. Total genome size
c. 6.5 kb. Genome unipartite; largest (or only) genome part 6.5 kb.
Genomic nucleic acid isolated by Peden and Symons (1973). Base composition 23.2
% G (CGMMV-W, 25.8% (CV4), 25.5% (CV3)); 24.6 % A (CGMMV-W, 25.8% (CV4), 25.8%
(CV3)); 20.6 % C (CGMMV-W, 19.3% (CV4), 18.3% (CV3)); 31.6 % U (CGMMV-W, 29.5%
(CV4), 30.8% (CV3)). Infectivity decreased when deproteinised with proteases;
retained when deproteinised with phenol or detergent. Poly A region absent.
Additional factor not required for infectivity. Genome has tRNA-like
activity. Genome accepts histidine. Nucleotide sequence references: Meshi et
Sequence database accession code(s)
Em(40)_vi:MCGSHCG Gb(84)_vi:MCGSHCG Cucumber green mottle mosaic virus (CGMMV)
complete genome. 10/92 6,421bp.
- J02054 Gb(84)_vi:MCGCPW cucumber green
mottle mosaic virus coat protein gene. 9/83 1,071bp.
Em(40)_vi:TOBMCG30 Gb(84)_vi:MCG30KP Cucumber green mottle mosaic virus 30K
protein gene, complete cds, and 180K and coat protein 3 sequences.
Features of the genome
Features of the genome: the
genomic RNA has been sequenced (Ugaki et al., 1991).
Non-genomic nucleic acid found in the virions; is subgenomic mRNA.
Sub-genomic mRNA found in infected cells.
Features of proteins
Virion protein(s) one;
Mr 17261; coat protein. Method of preparation: Fraenkel-Conrat (1957);
Tung and Knight (1972). Amino acid sequence: Meshi et al. (1983). Amino
acid composition: Nozu et al. (1971); Tung and Knight (1972).
Replication does not depend on a helper
Virions found in leaves, mesophyll,
epidermis, vascular parenchyma, xylem, phloem, companion cells and all parts of
the host plant; in cytoplasm and in cell vacuoles. Inclusions present in
infected cells; are crystals in the cytoplasm; they contain virions. Other
cellular changes: vesiculation of mitochondria.
Virus(es) with serologically related virions
Frangipani mosaic, kyuri green mottle mosaic, ribgrass mosaic,
tobacco mosaic, tomato mosaic and tobacco mild green mosaic viruses.
Virus(es) with serologically unrelated virions
Odontoglossum ringspot, sunn-hemp mosaic viruses.
Additional comments on relationships
The host ranges of cucumber green mottle mosaic virus and kyuri green
mottle mosaic virus are similar, and their coat proteins have greater amino acid
sequence similarity than either has to those of other tobamoviruses, which are
serologically more distantly related.
- Ainsworth, G.C. (1935).
Ann. appl. Biol. 22: 55.
- Fraenkel-Conrat, H. (1957).
Virology 4: 1.
- Francki, R.I.B. and McLean, G.D. (1968).
Aust. J. biol. Sci. 21: 1311.
- Francki, R.I.B., Hu, J. and
Palukaitis, P. (1986). Intervirology 26: 156.
- Hollings, M.,
Komuro, Y. and Tochihara, H. (1975). CMI/AAB Descr. Pl. Viruses No. 154,
- Meshi, T., Kiyama, R. Ohmi, T. and Okada, Y. (1983). Virology
- Nozu, Y., Tochihara, H., Komuro, Y. and Okada, Y. (1971).
Virology 45: 577.
- Peden, K.W.C. and Symons, R.H. (1973).
Virology 53: 487.
- Rao, D.C. and Varma, A. (1984).
Phytopath. Z. 109: 325.
- Tung, J.S. and Knight, C.A. (1972).
Virology 48: 574.
- Ugaki, M., Toriyama, M., Kakutani, T.,
Hidaka, S., Kiguchi, T., Nagata, R., Sato, T., Motoyoshi, F. and Nishiguchi, M.
(1991). J. gen. Virol. 72: 1487.
Cite this publication as:
Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.)
`Plant Viruses Online: Descriptions and Lists from the VIDE Database.
Version: 20th August 1996.' URL
Dallwitz, Paine and Zurcher (1993)
should also be cited.
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